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  • Bacterial Persistence

    Bacterial Persistence

    Inbunden - 2022 - Engelska - ISBN: 9781071616208

    This volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial cultures, strategies to enrich and re…

  • CRISPR

    CRISPR

    Inbunden - 2015 - Engelska - ISBN: 9781493926862

    This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that…

  • NF-kappa B

    NF-kappa B

    Inbunden - 2015 - Engelska - ISBN: 9781493924219

    Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-?B activation, regulation, and function. NF-kappa B: Methods and Protocols guides readers through standard approaches to detect NF-?B path…

  • Rab GTPases

    Rab GTPases

    Inbunden - 2021 - Engelska - ISBN: 9781071613450

    This second edition volume expands on the previous edition with a discussion of new research and discoveries in the Rab field. Chapters in this book cover topics such as new information on Rab regulation and localization; interaction; function; and…

  • Plant Circadian Networks

    Plant Circadian Networks

    Inbunden - 2021 - Engelska - ISBN: 9781071619117

    This second edition provides new and updated protocols to investigate clock-controlled parameters including period estimations and entrainment measurements and modelling. Chapters detail promoter activity using luciferase reporters, fluorescent-tag…

  • Enzyme Stabilization and Immobilization

    Enzyme Stabilization and Immobilization

    Inbunden - 2010 - Engelska - ISBN: 9781607618942

    In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods an…

  • Transplantation Immunology

    Transplantation Immunology

    Häftad - 2010 - Engelska - ISBN: 9781617376320

    Leading clinicians and scientists in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ. The authors present the latest techniques for HL…

  • Leucocytes

    Leucocytes

    Inbunden - 2012 - Engelska - ISBN: 9781617795268

    The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leuc…

  • Lipid Signaling Protocols

    Lipid Signaling Protocols

    Inbunden - 2008 - Engelska - ISBN: 9781588297273

    Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summa…

  • Lipid Signaling Protocols

    Lipid Signaling Protocols

    Häftad - 2014 - Engelska - ISBN: 9781627038829

    Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summa…

  • Bacterial Molecular Networks

    Bacterial Molecular Networks

    Häftad - 2016 - Engelska - ISBN: 9781493961566

    Network-based representations have become pervasive in most fields in biology. Bacterial Molecular Networks: Methods and Protocols provides authoritative descriptions of various experimental and computational methods enabling the characterization an…

  • T-Cell Trafficking

    T-Cell Trafficking

    Häftad - 2017 - Engelska - ISBN: 9781493957040

    In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the develop…

  • Serum/Plasma Proteomics

    Serum/Plasma Proteomics

    Häftad - 2016 - Engelska - ISBN: 9781493958016

    Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that all…

  • Chondrocytes

    Chondrocytes

    Häftad - 2021 - Engelska - ISBN: 9781071611210

    This detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the cho…

  • RNA Chaperones

    RNA Chaperones

    Häftad - 2020 - Engelska - ISBN: 9781071602331

    This book provides a wide spectrum of methods to study RNA chaperones in vitro, at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores…

  • Developmental Biology Protocols

    Developmental Biology Protocols

    Inbunden - 2000 - Engelska - ISBN: 9780896038530

    Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The…

  • Developmental Biology Protocols

    Developmental Biology Protocols

    Häftad - 2000 - Engelska - ISBN: 9780896035751

    Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The…

  • P-Type ATPases

    P-Type ATPases

    Inbunden - 2015 - Engelska - ISBN: 9781493931781

    This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all research…

  • Mammalian Oocyte Regulation

    Mammalian Oocyte Regulation

    Inbunden - 2012 - Engelska - ISBN: 9781627031905

    Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that tra…

  • Bioinformatics Methods and Protocols

    Bioinformatics Methods and Protocols

    Häftad - 2010 - Engelska - ISBN: 9781617371561

    Computers have become an essential component of modern biology. They help to manage the vast and increasing amount of biological data and continue to play an integral role in the discovery of new biological relationships. This in silico approach to…

  • Hedgehog Signaling Protocols

    Hedgehog Signaling Protocols

    Häftad - 2010 - Engelska - ISBN: 9781617377211

    Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental description, including anecdotal pointers and notes.

  • Apoptosis and Cancer

    Apoptosis and Cancer

    Häftad - 2010 - Engelska - ISBN: 9781617375828

    The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizin…

  • Microbial Systems Biology

    Microbial Systems Biology

    Inbunden - 2012 - Engelska - ISBN: 9781617798269

     Systems biology is the study of interactions between assorted components of biological systems with the aim of acquiring new insights into how organisms function and respond to different stimuli. Although more and more efforts are being directed to…

  • Agrobacterium Protocols

    Agrobacterium Protocols

    Häftad - 2016 - Engelska - ISBN: 9781493945412

    Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a te…

  • High Throughput Screening

    High Throughput Screening

    Häftad - 2011 - Engelska - ISBN: 9781617794919

    In the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche area of study to a major, essential scientific technique. In High Throughput Screening: Methods an…

  • Autoantibodies

    Autoantibodies

    Inbunden - 2018 - Engelska - ISBN: 9781493989485

    This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their…

  • PRINS and In Situ PCR Protocols

    PRINS and In Situ PCR Protocols

    Häftad - 2010 - Engelska - ISBN: 9781617376368

    The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen incre…

  • Flow Cytometry Protocols

    Flow Cytometry Protocols

    Häftad - 2016 - Engelska - ISBN: 9781493956623

    With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols…

  • Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols

    Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols

    Häftad - 2010 - Engelska - ISBN: 9781617376986

    This book provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA. Each chapter begins with a brief introduction to the method and purpose, and then goes into detailed protocols for every ste…

  • Protein Expression in Mammalian Cells

    Protein Expression in Mammalian Cells

    Inbunden - 2011 - Engelska - ISBN: 9781617793516

    Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and interven…

  • Targeted Drug Delivery

    Targeted Drug Delivery

    Häftad - 2019 - Engelska - ISBN: 9781493993574

    This volume looks at the latest techniques used to enable therapeutic molecules to be targeted for site-specific delivery. The chapters in this book discuss topics such as approaches to express biologically derived and synthetic nanocarriers; strate…

  • Nitrogen Fixation

    Nitrogen Fixation

    Inbunden - 2011 - Engelska - ISBN: 9781617791932

    Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent r…

  • Yeast Surface Display

    Yeast Surface Display

    Inbunden - 2015 - Engelska - ISBN: 9781493927470

    In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and…

  • Light Microscopy

    Light Microscopy

    Häftad - 2016 - Engelska - ISBN: 9781493957132

    Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrument…

  • Fasciola hepatica

    Fasciola hepatica

    Inbunden - 2020 - Engelska - ISBN: 9781071604748

    This volume described basic and advanced protocols to study F. hepatica parasite biology. Chapters guide readers through protocols on different developmental stages of F. hepatica, characterize tegumental, secreted proteins, spatial and temporal gen…

  • Fasciola hepatica

    Fasciola hepatica

    Häftad - 2021 - Engelska - ISBN: 9781071604779

    This volume described basic and advanced protocols to study F. hepatica parasite biology. Chapters guide readers through protocols on different developmental stages of F. hepatica, characterize tegumental, secreted proteins, spatial and temporal gen…

  • Poly(ADP-ribose) Polymerase

    Poly(ADP-ribose) Polymerase

    Inbunden - 2011 - Engelska - ISBN: 9781617792694

    Poly (ADP-ribose) Polymerases (PARPs) are abundant and ubiquitous proteins that regulate crucial processes of the cell cycle, DNA repair, genomic stability, and transcriptional regulation. Being involved in basic cell functions, PARPs mediate rapid…

  • Bacterial Chemosensing

    Bacterial Chemosensing

    Inbunden - 2018 - Engelska - ISBN: 9781493975761

    This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a…

  • Data Mining for Systems Biology

    Data Mining for Systems Biology

    Inbunden - 2012 - Engelska - ISBN: 9781627031066

    The post-genomic revolution is witnessing the generation of petabytes of data annually, with deep implications ranging across evolutionary theory, developmental biology, agriculture, and disease processes. Data Mining for Systems Biology: Methods an…

  • mRNA Decay

    mRNA Decay

    Häftad - 2018 - Engelska - ISBN: 9781493985159

    This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rate…

  • High-Throughput Screening Assays in Toxicology

    High-Throughput Screening Assays in Toxicology

    Inbunden - 2016 - Engelska - ISBN: 9781493963447

    This book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques. Divided into three sections, in vitro assays, in vivo assa…

  • T-Cell Motility

    T-Cell Motility

    Inbunden - 2019 - Engelska - ISBN: 9781493990351

    This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3…

  • Capillary Electrophoresis of Carbohydrates

    Capillary Electrophoresis of Carbohydrates

    Häftad - 2010 - Engelska - ISBN: 9781617372063

    Glycoconjugates such as glycoproteins and glycolipids play important roles in cell-cell interaction events, including development, differentiation, m- phogenesis, fertilization, inflammation, and metastasis. A number of reports have documented the a…

  • Directed Evolution Library Creation

    Directed Evolution Library Creation

    Häftad - 2010 - Engelska - ISBN: 9781617374715

    Biological systems are very special substrates for engineering—uniquely the products of evolution, they are easily redesigned by similar approaches. A simple algorithm of iterative cycles of diversification and selection, evolution works at al…

  • Zygotic Genome Activation

    Zygotic Genome Activation

    Häftad - 2018 - Engelska - ISBN: 9781493983599

    This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalia…

  • BCL-2 Family Proteins

    BCL-2 Family Proteins

    Inbunden - 2018 - Engelska - ISBN: 9781493988600

    This volume explores numerous techniques for the genetic, molecular, biochemical, and structural examination of BCL-2 family proteins and their interactions. The chapters in this book cover topics such as the relevance of BCL-2 proteins in health an…

  • Detection of Blotted Proteins

    Detection of Blotted Proteins

    Inbunden - 2015 - Engelska - ISBN: 9781493927173

    This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional t…

  • Epistasis

    Epistasis

    Häftad - 2016 - Engelska - ISBN: 9781493942756

    This volume presents a valuable and readily reproducible collection of established and emerging techniques on modern genetic analyses. Chapters focus on statistical or data mining analyses, genetic architecture, the burden of multiple testing, genet…

  • Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

    Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

    Inbunden - 2008 - Engelska - ISBN: 9781588297006

    From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of…

  • Phytochromes

    Phytochromes

    Inbunden - 2019 - Engelska - ISBN: 9781493996117

    This volume aims at providing readers with protocols to enhance their research for the next decade of phytochrome research. The chapters in this book cover topics such as tests for light-dependent interaction of phytochrome binding proteins using ye…

  • Phytochromes

    Phytochromes

    Häftad - 2020 - Engelska - ISBN: 9781493996148

    This volume aims at providing readers with protocols to enhance their research for the next decade of phytochrome research. The chapters in this book cover topics such as tests for light-dependent interaction of phytochrome binding proteins using ye…

  • Kidney Organogenesis

    Kidney Organogenesis

    Inbunden - 2019 - Engelska - ISBN: 9781493990207

    This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell,…

  • Protein Chromatography

    Protein Chromatography

    Inbunden - 2010 - Engelska - ISBN: 9781607619123

    A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native protei…

  • Brain Development

    Brain Development

    Häftad - 2016 - Engelska - ISBN: 9781493960255

    Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene e…