This book reveals not only how the eye evolved into an organ of vision, but also describes how molecular mechanisms of key molecules operate in the phototransduction cascade. In this groundbreaking text, experts also explain mechanisms for sensing r…
This volume provides a clear and detailed roadmap of how to design and execute a gene therapy experiment in order to obtain consistent results. Chapters in this book disseminate bits of unknown information that are important to consider during the c…
This second edition provides detailed LC-MS(/MS) procedures for the analysis of compounds of clinical and toxicological significance. Chapters detail new and updated methods for analyzing drugs focusing on advances in technology, alternate matrices,…
Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma. Only in the last 10-15 years has it become clear that astrocytes are multifunctional versatile cells that play key roles in a multitude of diver…
This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing. The volume includes a detailed introducto…
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for…
This book is a comprehensive and richly-illustrated guide to cardiac CT, its current state, applications, and future directions. While the first edition of this text focused on what was then a novel instrument looking for application, this edition c…
"This volume explores numerous techniques used to study the ubiquitin proteasome system. The chapters in this book are organized into five parts and cover topics such as determining the mechanisms of action for E2s, E3s, and DUB enzymes; the latest…
This volume presents detailed laboratory procedures in an easy to follow format that can be carried out with success by investigators lacking previous exposure to a specific research method. Chapter guide readers through the application of molecular…
This volume details cutting-edge protocols on the characterization of the genome, epigenome, proteome, metabolome and single-cell transcriptome of tumors and tumor-derived cultures. Chapters focus on subpopulations of cells with stem-like properties…
During the last two decades, many new techniques and devices have appeared for measuring blood pressure both directly and indi- rectly. At present, there is no single source for this information; nor is there information on the accuracy and sources…
Leading clinicians and scientists in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ. The authors present the latest techniques for HL…
The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leuc…
This book came about as a result of a review I had written earlier on fea tures of cellular changes occurring during anuran metamorphosis. Only a limited treatment of this subject was possible in such a circumscribed work and only specific examples…
Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summa…
Network-based representations have become pervasive in most fields in biology. Bacterial Molecular Networks: Methods and Protocols provides authoritative descriptions of various experimental and computational methods enabling the characterization an…
In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the develop…
NanoBiotechnology is a groundbreaking text investigating the recent advances and future direction of nanobiotechnology. It will assist scientists and students in learning the fundamentals and cutting-edge nature of this new and emerging science. Foc…
Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that all…
Itisonlyrecently thatthe naturaloccurrenceoffree radicalsin biological tissue has become widely accepted, and that the suspi- cion with which biologists previously viewed the free radicals of radiationchemistryhas beenplacedin a broaderperspective.…
These comprehensive yet concise collections of articles by world experts survey the latest findings on the molecular biology of diabetes and insulin action and synthesize a coherent understanding of the subject. Topics include the etiology of type I…
This volume represents a compendium of scientific findings and approaches to the study of angiogenesis in cancer. The second edition of Antiangiogenic Agents in Cancer Therapy is intended to give a current perspective on the state-of-the-art of angi…
Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events-changes in form, structure, and function of the org- ism. The other…
This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all research…
Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that tra…
In the last few years, technologies that allow for monitoring and manipulating neuronal networks at a single cell resolution have been rapidly expanding. In Neuronal Network Analysis: Concepts and Experimental Approaches, experts in the field provid…
Computers have become an essential component of modern biology. They help to manage the vast and increasing amount of biological data and continue to play an integral role in the discovery of new biological relationships. This in silico approach to…
In recent years there have been huge advances in the understanding of the genetic and molecular basis of the fibrocystic diseases. This volume provides a thorough review of fibrocyctic diseases that affect the liver. It contains in-depth discussions…
With the loss of work days, the price of health care and payments for compensation, litigation, and malpractice, and the overwhelming cost of human suffering, chronic pain syndromes affect humanity enormously on both an economic and personal level.…
Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental description, including anecdotal pointers and notes.
The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizin…
Systems biology is the study of interactions between assorted components of biological systems with the aim of acquiring new insights into how organisms function and respond to different stimuli. Although more and more efforts are being directed to…
The last two decades have seen prodigious growth in the application of brain imaging methods to questions of substance abuse and addiction. Despite considerable advances in our understanding of the central effects of drugs provided by preclinical da…
In the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche area of study to a major, essential scientific technique. In High Throughput Screening: Methods an…
This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their…
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen incre…
LA WRENCE G. RAISZ, MD Division of Endocrinology and Metabolism, University of Connecticut Health Center. Farmington. CT The rapid transfer of new knowledge concerning the pathogenesis, diagnosis, preven- tion, and treatment of disease into clinical…
With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols…
Involved in nearly every therapeutic area, particularly cancer, biomarkers have experienced tremendous advances since the first edition of this book, both in the discovery of biomarkers and in their applications. To aid in this imperative research,…
This book provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA. Each chapter begins with a brief introduction to the method and purpose, and then goes into detailed protocols for every ste…
Recent years have seen remarkable advances in the devel- ment of techniques that have direct applications in neurological research. In consequence, the circulatory and metabolic status of the brain can be measured and correlated with changes m struc…
Secondary forms of hypertension are not uncommon in clinical prac- tice, but they are often overlooked or forgotten by clinicians in many fields of medicine. Dr. George Mansoor' s volume on Secondary Hyper- tension is an important contribution to th…
Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and interven…
This volume looks at the latest techniques used to enable therapeutic molecules to be targeted for site-specific delivery. The chapters in this book discuss topics such as approaches to express biologically derived and synthetic nanocarriers; strate…
Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent r…
The volume aims at providing an overview of cutting-edge methods and protocols used in neuroscience and neurological research. Neurotransmitter Transporters: Investigative Methods will be of interest to scientists, graduate students, and advanced un…
In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and…
Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrument…
Poly (ADP-ribose) Polymerases (PARPs) are abundant and ubiquitous proteins that regulate crucial processes of the cell cycle, DNA repair, genomic stability, and transcriptional regulation. Being involved in basic cell functions, PARPs mediate rapid…
Studies on Men's Health and Fertility provides a comprehensive series of up-to-the-minute reviews addressing the role of oxidative stress in the aetiology of reproductive pathologies in the male. This volume represents by far the most detailed, auth…
